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KMID : 0545120030130040578
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Journal of Microbiology and Biotechnology
2003 Volume.13 No. 4 p.578 ~ p.583
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Desulfurization of Dibenzothiophene and Diesel Oil by Metabolically Engineered Escherichia coli
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PARK, SI JAE
LEE, IN-SU/CHANG, YONG KEUN/LEE, SANG YUP
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Abstract
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The desulfurization genes (dszABC ) were cloned from Gordonia nitida. Nucleotide sequences similarity between the dszABC genes of G. nitida and those of Rhoducoccus rhodochrous IGTS8 was 89%. The similarities of deduced amino acids between the two were 86% for DszA, 86% for DszB, and 90% for DszC. The G. nitida dszABC genes were expressed in several different Escherichia coli strains under an inducible tre prumoter. Cultivation of these metabolically engineered E. coli strains in the presence of 0.2 mM dibenzothiophene (DBT) allowed the conversion of DBT to 2-hydroxybiphenyl (2-HBP), which is the final metaholite of the sulfur-specific desulfuriration pathway. The maximum conversion of DBT to 2-HBP was 16% in 60 h. Recombinant E. coli was applied for the deep desulfuriration of diesel oil supplemented into the medium at 5% (v/v). Sulfur content in diesel oil was decreased from 250 ㎎ sulfur/l to 212.5 ㎎ sulfur/ l, resulting in the removal of 15% of sulfur in diesel oil in 60 h.
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